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. 2008 May 7;3(5):e2127. doi: 10.1371/journal.pone.0002127

Figure 2. Enhanced cAMP reporter, expressed in tissues of double transgenic CMV-rtTA/pBI-cAMP mice.

Figure 2

A. Transgenic construct in pBI vector, with C-YFP and RII-CFP in opposing orientations around a bi-directional tetracycline-inducible promoter. Genotyping primers (▸,◂) and the resulting PCR products are indicated. B. Example of genotyping on genomic DNA (gDNA) from a mouse lacking (non-Tg) or possessing the integrated transgene (pBI-cAMP Tg). PCRs with each template tested for RII-CFP (lanes 1, 4, 7), C-YFP (lanes 2, 5, 8) and an endogenous gene, PLCβ2 (lanes 3, 6, 9). C–E. Tissues from double transgenic CMV-rtTA/pBI-cAMP mice were immunostained with anti-GFP (green) to visualize the reporter in skeletal myofibers (C), cardiac myocytes (D) and pancreas (E). In the pancreas, only acinar cells express the reporter, while islets of Langerhans (immunostained with anti-insulin, red) do not. In D., nuclei are counterstained red with TO-PRO-3. Scale bars, 50 µm.