Figure 4.

Effects of FBXL genes on Hoxb9 promoter activity in HeLa cells. (A) Effects of siRNAs on FBXL expression. We designed siRNAs—iRhF10 and iRhF11—and used RT–PCR to examine the effects of these siRNAs on Hoxb9 promoter activity. Specific siRNAs against FBXL10 increased promoter activity, whereas siRNAs specifically against FBXL11 either did not increase or weakly increased promoter activity. (B) Treatment with siRNAs influences intrinsic FBXL expression. Each of these siRNAs down-regulated FBXL expression, as evident by the definitive reduction of RT–PCR signals. RT–PCR samples were normalized relative to β-actin expression.