Figure 6.

Assessment of domains involved in the specific regulation of Hoxb9. (A) Schematic diagrams of Fbxl10 mutants. ΔJ, C and ΔP represent deletion mutants of the jmjC domain, cxxc domain, and the PHD domain, respectively. ΔCP represents a deletion mutant of both cxxc and PHD domains. Three other mutants, pmJ, pmC, pmP and pmCP, have point mutations in jmjC, cxxc, PHD and both the cxxc and PHD domains, respectively. (B) Subcellular localization of jcp1 mutants. Each mutant protein was fused with an HA-tag at C-terminal and then expressed in HeLa cells. Fusion proteins were localized by immunocytochemistry with anti-HA antibody. All mutants localized to the nucleus. (C) Effect of jcp1 mutants on Hoxb9 promoter activity. We co-transfected HeLa cells with mutant-expressing plasmids and Hoxb9 luciferase reporter plasmids and examined the influence of each mutation by measuring luciferase activity.