Figure 2.

C-terminal diversity of p53 and p73 isoforms influences sequence-specific DNA binding. (A) EMSA showing binding of in vitro translated p53 and p73α, β, γ and δ to a double-stranded, ³²P labeled oligonucleotide containing a consensus p53-binding site (5 nM). A 200-fold molar excess of the same oligonucleotide without ³²P label was used as a specific competitor, a scrambled oligonucleotide as an unspecific competitor. 200 ng anti-HA antibody was added for supershift analysis as indicated. 200 ng anti-p53 (PAb421) was added to block the p53 CTD. (B) Western Blot demonstrating equal amounts of all in vitro translated proteins. Immunodetection was performed with an anti-HA antibody.