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. 2008 Feb 11;36(6):1900–1912. doi: 10.1093/nar/gkn044

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Influence of p53 CTD on intranuclear mobility. (A) Fluorescence micrographs of H1299 cells transfected to express YFP fusion proteins of p53, p53Δ30, p73 and p73 + 30. Scale bar, 10 µm. (B) Fluorescence recovery after photobleaching (FRAP). H1299 cells were transfected with YFP fusion proteins. Shown are fluorescence recovery curves calculated from sequential scanned images recorded after photobleaching of a 2.56 µm wide strip for 1 s. Examples of the bleached areas are shown in (A). Fluorescence intensity before photobleaching was set to 100%. Each data point represents the mean ± SE of n cells. Recovery curves of immobile histone H2B and highly mobile free YFP are shown for comparison. (C) Representative FRAP data from individual cells fitted to the free-diffusion model (red curves) and the extended reaction-diffusion model (blue curves) according to Hinow et al. (41). P value, confidence level of improvement by the extended model.