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. 2008 Feb 14;36(6):2060–2072. doi: 10.1093/nar/gkn049

Figure 5.

Figure 5.

Residues 211–384 of RAG1 stabilize Ku70/Ku80 association with a RAG–RSS complex. (A) Diagram of RAG1 NTD truncation mutants used in these experiments, labeled as described in Figure 3. (B) EMSA of RAG1 NTD truncation mutant protein preparations. WT cMR1, FLMR1 or the RAG1 NTD mutants shown in (A) were co-expressed with, or mutant forms of 181MR1 were co-expressed with cMR2 in 293 cells and purified using the mild protocol. Radiolabeled intact 12-RSS substrate was incubated with HMGB1 and these various RAG preparations and protein–DNA complexes were subjected to supershift analysis by EMSA using a monoclonal anti-Ku80 antibody as indicated above the gel.