Figure 6.

Identification of additional cleavage sites of endonuclease SegB located in the phage T2L tRNA gene region. Images of the DNA sequencing gels of the upper strand of indicated tRNA genes are presented. The labeled extended DNA was incubated without (lane E) or with endonuclease SegB (lane 1), or with endonuclease SegB followed by DNA extension with Klenow fragment to determine the cleavage points in the lower strand (lane 2). Products of the four DNA sequencing reactions designated as G, A, T and C that were generated with the same primer were used as the ladders. The analyses were carried out as described in ‘Materials and Methods’ section. The sequences surrounding the cleavage sites are shown; positions of the DNA breaks in the upper and lower strands are indicated with closed and open arrowheads, respectively; the major strand break points are circled.