Table 1.

attP docking sites characterized with the UAS::luciferase reporter^a

Site	Chr.	Cytology	Location relative to nearest genes
attP1b	2R	56C1	Intergenic: flanked by sbbcand IM23
attP2b	3L	68A4	Intergenic: flanked by CG6310 and MocsI
attP3	X	19C4	Intergenic: flanked by CG1631 and CG15462
attP4	X	12C6	Intragenic: inside the 5′ UTR of Clic
attP10	3	85D7/92B1	TEd
attP14	2L	36A10	Intragenic: inside an intron of grp
attP16	2R	53C4	Intergenic: flanked by CG15711 and CG33960
attP18	X	6C12	Intragenic: inside the 5′ UTR of CG14438
attP22	2R	45D8	Intergenic: flanked by ced-6e and CG13952
attP23	2R	60C7	Intragenic: inside an intron of bs
attP24f	2R	42C1	Intergenic: flanked by Or42A and Tsp42A
	2R	58C1	Intergenic: flanked by CG34205 and a
attP29	2L	21E2	Intragenic: inside the 5′ UTR of drongo
attP30	2L	29C3	Intragenic: inside an intron on Akap200
attP32	2R	49D6	Intergenic: flanked by CG17574 and bic
attP33	2R	50B6	Intergenic: flanked by CG12464 and fas
attP40	2L	25C7	Intergenic: flanked by CG14035 and Msp-300
attP52	3R	89B11	Intragenic: inside the 5′ UTR/intron of gishg
attP64	3R	89B9	Intragenic: inside the 5′ UTR/intron of tarag
attP83	CyO	39D3	Intergenic: flanked by nrv3 and His1h
attP88	3L	64A12	Intragenic: inside a coding exon of CG1265
attP112	3L	68C13	Intragenic: inside an intron of MobI
attP154	3R	97D2	Intergenic: flanked by CG14247 and Tl

^aBased on release 5.1 of the D. melanogaster genome.

^bSee reference ²².

^cBased on accession AF247562.

^dTransposable Element, RT1-alpha; attP10 maps genetically to chromosome 3, butby iPCR it is unclear if it is in the RT1-alpha element at 85D7 or 92B1.

^eThe 5′ end of ced-6 is based on RE47146.

^fPCR results indicate that the UAS-luciferase reporter integrated at 42C1 but not 58C1.

^gOn the basis of alternative splicing, the attP docking site is located in the 5′ UTR of some transcripts and in the intron of others.

^hOn wild-type chromosome 2, position 39D3 is flanked by nrv3 and His1; we did not determine if this gene order holds on CyO.