Figure 6.

Restoration of CAM-deficient phenotype by sugar feeding of detached leaves. Leaves of wild type (WT) and CAM-deficient lines 351 and 371 were detached from plants that had been treated with 0.3 m NaCl for 10 d and then placed in water (control) or fed with 0.2 m Suc or mannitol for the latter 8 h of the photoperiod. Measurements of the change (Δ) in leaf titratable acidity at the start and end of the subsequent dark period were determined and plotted as the difference between the two measurements. Each point is the mean of five replicates ± se. Student's t test P value for control between WT and 351: P = 5.4 × 10⁻⁴** and WT and 371: P = 3.3 × 10⁻⁴**. Student's t test P value for Suc feeding between WT and 351: P = 1.4 × 10⁻²* and WT and 371: P = 1.8 × 10⁻²*. Student's t test P value for mannitol feeding between WT and 351: P = 2.0 × 10⁻⁴** and WT and 371: P = 1.2 × 10⁻⁶**.