Figure 1.

A, GmosAAP1-mediated [¹⁴C]Pro uptake at different substrate concentrations. The experiments were performed at pH 4.5. The values represent means of three independent experiments ± sd. B, pH dependence of the uptake rate of [¹⁴C]Pro in the yeast mutant 22Δ8AA expressing GmosAAP1. Yeast expressing GmosAAP1 in pDR196 were measured at different pH values and an 18.8 μm substrate concentration. The values represent means of three independent experiments ± sd. C, Influence of plasma membrane energization on the uptake rate of [¹⁴C]Pro in the yeast mutant 22Δ8AA expressing GmosAAP1. The yeast cells were preincubated for 5 min in the presence of 100 mm Glc (control), without Glc, or with Glc and 0.1 mm 2,4-dinitrophenol (DNP), 0.1 mm diethylstilbestrol (DES), 0.1 mm carbonyl cyanide m-chlorophenylhydrazone (CCCP), or 0.1 mm vanadate. The values represent means of three independent experiments ± sd.