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. 2008 May;147(1):331–339. doi: 10.1104/pp.108.117572

Figure 4.

Figure 4.

ManNAc-6-P production in vitro and in planta by GNE expression in Arabidopsis seedling cultures. A, Determination of ManNAc-6-P production in vitro. MS/MS spectra of the HexNAc-6-P peak region acquired from the GlcNAc-6-P standard (S), from extracts of 35S:GNE transgenic plants (GNE), and from wild-type plants (wt) incubated in the presence of UDP-GlcNAc and ATP. Masses of fragments arising from AA-labeled HexNAc-6-P are underlined. B, Demonstration of ManNAc-6-P production in planta. The presence of HexNAc-6-P in Arabidopsis extracts was analyzed by direct infusion ESI-MS/MS analysis of the [M−H]− parent ion of mass 300.0. Characteristic fragmentation products are labeled F1, F2, and F3. Spectra were obtained with GlcNAc-6-P standard (S) and extracts from 35S:GNE transgenic plants (GNE) and wild-type plants (wt).