Figure 7.
In planta synthesis of CMP-Neu5Ac. A, LC-ESI-MS/MS analysis. CMP-Neu5Ac standard (S), extract from wild-type control (wt), and extract from GNE-, NANS-, and CMAS-expressing transgenic Arabidopsis (GNE NANS CMAS) were analyzed with parent ion mass set to 613.1 D. B, Mass spectra at the elution position of CMP-Neu5Ac: standard CMP-Neu5Ac (S), wild-type control (wt), and GNE-, NANS-, and CMAS-expressing transgenic Arabidopsis (GNE NANS CMAS). All runs were set to identical absolute intensity.