Figure 8.

Analysis of changes in overall N-glycosylation in Neu5Ac- and CMP-Neu5Ac-producing Arabidopsis plants. A, Proteins were extracted from leaves and subjected to SDS-PAGE under reducing conditions. Immunoblotting was performed using anti-horseradish peroxidase antibodies. Lane 1, Arabidopsis wild type; lane 2, xylt fuct mutant plant (negative control; Strasser et al., 2004); lane 3, GNE NANS plant; lane 4, GNE NANS CMAS plant. The bottom panel shows Ponceau S staining of the membrane. B, MALDI-TOF-MS analysis of total N-glycans present in leaves from wild-type (wt), GNE NANS, and GNE NANS CMAS Arabidopsis plants. For abbreviations of N-glycan structures, see http://www.proglycan.com/.