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. 2008 May;147(1):216–227. doi: 10.1104/pp.108.116137

Figure 1.

Figure 1.

ARS activity and chlorophyll in the ars mutants. A, ARS activity on solid medium. Cells were streaked onto the surface of solid TAP-S medium and grown for several days before ARS activity was assayed. ARS activity is detected as a blue halo surrounding the colonies. B, ARS activity in liquid medium. ARS activity was measured after 24 h of S starvation. The data are averages of three independent experiments. C, Loss of chlorophyll in S-starved cells. Chlorophyll a and b content was normalized to the value on day 0 for each strain. Values represent means of three independent experiments. Strains used are D66 (parental strain), ars11 and ars44 (snrk2.1−), tars11#34 (ars11 complemented with SNRK2.1), sac1 (sac1 mutant), sac3 (sac3 mutant), and 11-3#16 (ars11 sac3 double mutant).