In situ hybridization of HvHAK1 mRNA in barley roots. Cross sections of seminal barley roots located 10 to 20 mm from the tip are shown. The expression of HvHAK1 is indicated by dark granules. A, Antisense probe. B, Sense probe. C, cortex; E, endodermis; VC, vascular cylinder. Bars, 100 μm.