Figure 5.

Rb⁺ uptake is enhanced by K⁺ starvation in HvHAK1-expressing yeast cells. A, Rb⁺ uptake from a 100 μm Rb⁺ solution by HvHAK1-expressing yeast cells grown overnight at 30 mm KCl and deprived of K⁺ (no K⁺ added) for different time periods (n = 6). B, Reciprocal experiment (n = 5) with 210-min K⁺-deprived cells transferred to a 30 mm KCl medium. C, Rb⁺ uptake (n = 6) from a wide range of micromolar Rb⁺ concentrations for K⁺-starved cells (white triangles) and K⁺-nonstarved cells (black triangles). D, Effect of K⁺ deprivation on the accumulation of HvHAK1 transcripts in yeast cells (n = 7). Error bars represent se.