Figure 8.

The PPZ1 phosphatase and the HAL4/5 kinases control the inducible transport of Rb⁺ mediated by HvHAK1 in yeast cells. A, Rb⁺ uptake from a 100 μm Rb⁺ solution measured in K⁺-starved (210 min) and K⁺-nonstarved trk1Δtrk2Δ and trk1Δtrk2Δhal4Δhal5Δ HvHAK1-expressing yeast cells (n = 5). B, Under the same experimental conditions, Rb⁺ uptake was also measured in trk1Δtrk2Δ and trk1Δtrk2Δppz1Δ HvHAK1-expressing yeast cells derived from the DBY746 strain (n = 5). C and D, Accumulation of the DiOC6 fluorescent dye for cells grown in the presence or absence of K⁺ (n = 3). Error bars represent se. Different letters indicate significantly different values (P ≤ 0.01 for A and D, P < 0.001 for B and C).