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. 2008 May;147(1):128–142. doi: 10.1104/pp.107.111799

Figure 3.

Figure 3.

RPL23aB with an N-terminal GFP5 tag is unable to accumulate in the nucleolus in tobacco epidermal cells. A to D, CLSM images of tobacco epidermal cells transiently coexpressing GFP5-RPL23aA and free mRFP (A and B) or GFP5-RPL23aB and free mRFP (C and D). E and F, Close-up images of cells expressing GFP5-RPL23aA (E) or GFP5-RPL23aB (F). Solid white arrowheads point to nucleoli (A, B, and E) and transparent white arrowheads point to nucleolar exclusion zones (C and F). Free mRFP delineates the cytoplasm and nucleoplasm (A–D, middle). Images of the same optical slice were merged to show signal overlap (A–D, right). Bars = 10 μm. [See online article for color version of this figure.]