The effect of daylength and temperature on in vivo P700 parameters in P. banksiana needles grown under different daylength and temperature regimes. After a steady-state level of P700+ was achieved under FR illumination, single-turnover and multiple-turnover pulses were applied. A, Steady-state oxidation of P700 (ΔA820/A820). B, Reduction kinetics of P700+ after the FR light was turned off (t/2). C, Effective intersystem electron pool size (e−/P700). All measurements were performed at the growth temperature. Each data point represents the average of n = 6 to 8 ± se biological replicates. •, ▪, and * indicate significant differences due to daylength, temperature, and their interactive effect, respectively. One symbol, P < 0.05; two symbols, P < 0.01; three symbols, P < 0.001.