Figure 6.

Antifreeze activity in the culture medium of wild-type bromegrass cells grown for 7 d at 25°C (A), 10°C (B), and 4°C (C) compared to antifreeze activity in the culture medium of transgenic bromegrass cell lines T6 grown for 7 d at 25°C (D), 10°C (E), and 4°C (F) and T2 grown for 7 d at 25°C (G). H, Antifreeze activity of recombinant BiCHT1 proteins (tag-removed). All the culture media were concentrated 50-fold for detection of antifreeze activity. Approximate concentrations of BiCHT1 proteins (mg/mL) were 0.1 (A), 0.14 (B), 0.04 (C), 0.35 (D), 0.3 (E), 0.2 (F), 0.25 (G), and 2.0 (H). Bars = 50 μm.