Figure 8.

Expression of BiCHT1 proteins in the culture medium of wild-type bromegrass cells grown at 25°C. Cultures were initiated by inoculating 1.5 g of wild-type bromegrass cells. Coomassie Brilliant Blue-stained SDS-PAGE gels (lanes 1–5) showing expression of BiCHT1 protein (arrowed) in the culture medium and their corresponding western-blot images using anti-CHT3 antibody (lanes 6–10). Culture media were prepared from cultures incubated for a day (lanes 1, 6), 3 d (lanes 2, 7), 7 d (lanes 3, 8), 10 d (lanes 4, 9), and 14 d (lanes 5, 10) after inoculation. Twenty microliters of a 50-fold concentrated culture medium was applied to each lane.