Figure 2.

Auxin and NO induce the accumulation of PA, PIP, and PIP₂. Cucumber explants were labeled with 15 μCi per explant for 16 h and subsequently treated for 10 min with buffer (C = control), the auxins NAA or IAA, or the NO donor SNAP as indicated. PLs were extracted and separated by TLC using an alkaline (A) or an EtAc (B) solvent system. Radioactivity was visualized by autoradiograph and quantified from nonoverexposed autoradiographs (Supplemental Fig. S1) using ImageJ software. C, Standardization was carried out by expressing the results as a ratio: labeled PA, PIP, or PIP₂ to total label in PLs. Values were subsequently expressed as fold accumulation ± se by dividing the ratio in a treatment by that in the corresponding control, taking the level of the control as 1. Data represent five independent experiments. Bars with asterisks are significantly different (t test, P < 0.05) from the control treatment.