Figure 3.

PA, PIP, and PIP₂ accumulate rapidly and transiently upon treatment of cucumber explants with auxin and NO. Explants labeled as in Figure 2 were incubated with buffer (C = control), 10 μm IAA, or 500 μm SNAP for different times as indicated. PLs were extracted and separated by TLC using an alkaline (A) or an EtAc (C) solvent system. Radioactivity was visualized by autoradiograph and quantified as described in Figure 2. B, Autoradiograph from a longer exposure of the chromatograph shown in A demonstrating the increase in PIP₂. D, Results are expressed as fold accumulation ± se taking the control values as 1. Data represent three independent experiments. Values with asterisks are significantly different (t test, P < 0.05) from the control treatment.