Figure 5.

PLD activation is involved in the auxin- and NO-induced PA accumulation. Cucumber explants were labeled as in Figure 2, and in vivo PLD activity was measured by treating them for 10 min with buffer (C = control), 10 μm IAA, or 500 μm SNAP in the presence of 0.5% (v/v) 1-butanol. A, Lipids were separated by TLC using an EtAc solvent system to separate PBut and PA from the other PLs. B, Autoradiograph from a longer exposure of the chromatograph shown in A demonstrating the increase in PBut. C, Results from three independent experiments are expressed as fold accumulation ± se taking the control values as 1. Bars with asterisks are significantly different (t test, P < 0.05) from the control treatment. Values are different from PA fold accumulation in the presence of butanol.