Figure 4.

Effects of increasing concentrations (0–1 m) of UDP-Xyl, UDP-Arap, or UDP-GlcA on the incorporation of [¹⁴C]Xyl or [¹⁴C]Ara into ethanol-insoluble products by wheat Golgi-enriched membranes. A, Effect of increasing amounts of UDP-Xyl on [¹⁴C]Ara transfer from UDP-[¹⁴C]Ara (0.2 mm, 3.5 cpm pmol⁻¹). B, Effect of increasing amounts of UDP-Arap on [¹⁴C]Xyl transfer from UDP-[¹⁴C]Xyl (0.5 mm, 3.5 cpm pmol⁻¹). C, Effect of increasing amounts of UDP-GlcA on [¹⁴C]Xyl transfer from UDP-[¹⁴C]Xyl (0.5 mm, 3.5 cpm pmol⁻¹). In all reactions, the ¹⁴C-radiolabel incorporation was monitored as cpm per reaction, and the assays were carried out two times.