Figure 7.

Sepharose-CL-6B fractionation profiles (A) of the partially purified GAX from wheat seedling cell walls (before and after treatment with endo-xylanase III) along with the [¹⁴C]GlcA-labeled product 4 (from 1- and 16-h incubation reactions), and Bio-gel P2 fractionation profiles (B) of ¹⁴C-radiolabed product 1 (top), product 2 (middle), and product 4 (bottom) prepared in vitro by wheat Golgi-enriched membranes (see Table II). The Sepharose-CL-6B column was eluted with 0.1 mm NaOH containing 0.01% NaBH₄, and 1-mL fractions were collected. The elution volumes of dextrans (500 and 40 kD), BSA (66 kD), and Xyl are indicated with arrows at the top as Vo, ∼66 kD, 40 kD, and total elution volume (Vt), respectively. The Bio-gel P2 column was eluted as described in “Materials and Methods.” [See online article for color version of this figure.]