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Effects of nucleotide sugars (0.5 mm) on [14C]GlcA transfer from UDP-[14C]GlcA (2 μm, 90,000 cpm reaction−1) into ethanol-insoluble products
Reactions were conducted in triplicate.
| Nucleotide Sugar Added | GlcA Transfer into Ethanol-Insoluble Product | |
|---|---|---|
| pmol reaction−1 | % of control | |
| None | 1.1 ± 0.06 | 12 |
| UDP-Xyl (control) | 8.9 ± 0.18 | 100 |
| UDP-Glc | 4.5 ± 0.15 | 50 |
| GDP-Man | 3.4 ± 0.15 | 38 |
| UDP-Gal | 3.6 ± 0.18 | 40 |
| UDP-GalNAc | 2.1 ± 0.12 | 23 |
| GDP-Fuc | 3.9 ± 0.15 | 44 |
