. 2008 May;147(1):78–91. doi: 10.1104/pp.107.115576

Table II.

Incorporation of ¹⁴C-radiolabel into ethanol-insoluble products by wheat Golgi-enriched membranes and its distribution among monosaccharides after total acid hydrolysis of the products and HPAEC analysis

Products 1 and 2 were obtained from the incubation of microsomes with 0.5 mm UDP-[¹⁴C]Xyl (approximately 3.5 cpm pmol⁻¹) in the absence or presence, respectively, of 0.2 mm UDP-[¹⁴C]Arap (approximately 3.5 cpm pmol⁻¹). Product 3 was prepared by incubation with 0.5 mm UDP-[¹⁴C]Xyl and UDP-[¹⁴C]GlcA (2 μm, approximately 900 cpm pmol⁻¹), and product 4 was prepared by incubation with all three of these UDP-¹⁴C-labeled sugars. Incubation times were 90 min, and each reaction was conducted in triplicate. ND, Not detected.

Product	Total ¹⁴C-Radiolabel Incorporated	Monosaccharide Incorporation Rate
Product	Total ¹⁴C-Radiolabel Incorporated	Xyl	Ara	GlcA
	cpm reaction⁻¹	pmol min⁻¹ mg⁻¹ protein
Product 1	2,500 ± 500	26.5 ± 0.1	12.0 ± 0.5	ND
Product 2	4,150 ± 600	44.5 ± 0.2	21.7 ± 0.6	ND
Product 3	15,000 ± 1,000	73.0 ± 0.8	19.3 ± 0.7	0.64 ± 0.01
Product 4	16,500 ± 1,500	65.7 ± 1.5	21.6 ± 0.8	0.70 ± 0.01