Table 2.

Catalytic and hapten binding properties of hu17E8 and variants

Clone	kcat, min−1	Km, μM	kcat/Km, M−1⋅min−1	kcat/Km (mutant)	EC50 (hu17E8)
				kcat/Km (hu17E8)	EC50 (mutant)
hu17E8	59	580	1.0  ×  105	1.0	1.0
1.1	—*	—*	<5.0  ×  103	<0.05	0.11
1.4	36	2,700	1.3  ×  104	0.13	0.050
1.10	—*	—*	<5.0  ×  103	<0.05	4.8
2.1	34	930	3.7  ×  104	0.37	4.0
2.2	—*	—*	<5.0  ×  103	<0.05	3.0
2.3	96	2,400	4.0  ×  104	0.40	2.5
2.5	7.1	800	9.0  ×  103	0.09	1.9
3.1	110	530	2.0  ×  105	2.0	0.52
3.2	46	1,600	3.0  ×  104	0.3	0.16
2.21	—*	—*	<5.0  ×  103	<0.05	7.5

^*Where catalytic activities were too weak to determine k_cat and K_m, k_cat/K_m was conservatively estimated as less than 5,000 M⁻¹⋅min⁻¹.