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Catalytic properties of hu17E8 active-site mutants
| Mutation | kcat, min−1 | Km, μM | kcat/Km, M−1⋅min−1 |
kcat/Km (mutant)
|
EC50 (hu17E8)
|
|---|---|---|---|---|---|
| kcat/Km (hu17E8) | EC50 (mutant) | ||||
| hu17E8 | 59 | 580 | 1.0 × 105 | 1.0 | 1.0 |
| His-35HAla | —* | —* | <5.0 × 103 | <0.05 | ND |
| His-35HGln | 30 | 3,800 | 8.0 × 103 | 0.08 | 2.1 |
| Ser-95HAla | 44 | 210 | 2.1 × 105 | 2.1 | 1.4 |
ND = not determined.
Activity too weak to determine kcat and Km.
