Table 2.

Summary of the effects of PMA on 8cpt-cAMP-induced responses in EBV-transformed lymphocytes

	Basal, nS	8cpt-cAMP, nS	8cpt-cAMP + PMA, nS
Wild-type
Absence of chelerythrine	0.40 ± 0.14 (12)	1.42 ± 0.59 (12)	0.38 ± 0.16* (9)
With chelerythrine	0.32 ± 0.20 (4)	1.33 ± 0.41 (4)	1.10 ± 0.44 (4)
Heterozygous (overall, 24)	0.46 ± 0.14	3.03 ± 1.38	1.99 ± 1.65*
Wild-type-like (5)	0.51 ± 0.18	1.42 ± 0.38	0.54 ± 0.19*
“Intermediate” (14)	0.44 ± 0.13	2.93 ± 0.78	1.51 ± 0.64*
Homozygous-like (5)	0.47 ± 0.07	4.92 ± 1.06	4.79 ± 1.04
Homozygous (16)	0.52 ± 0.17	4.71 ± 1.28	4.60 ± 1.27

In the wild-type group, the presence of chelerythrine in the pipet solution significantly reduced the inhibition by PMA (P < 0.001) but had no effect on either the basal conductance or the response to 8cpt-cAMP. The values obtained for PMA are significantly different from the slope conductance values obtained with 8cpt-cyclic AMP alone with the exception of the homozygous-like and homozygous variant: ∗, P < 0.05. 8cpt-cAMP was applied intracellularly via the recording pipet.