Table 2.

Binding kinetics for Z38 and Z34C

	koff, ×10−3 (s−1)	kon, ×105 (M−1⋅s−1)	Kd (nM)
Z38	40.6 ± 0.8	2.20 ± 0.18	185 ± 15
Z34C	30.2 ± 0.2	15.1 ± 0.6	20.0 ± 0.8
Z-domain	6.81 ± 0.01	4.56 ± 0.02	14.9 ± 0.1

Surface plasmon resonance is described in Materials and Methods. Errors reflect the standard deviations from two individual measurements using the same biosensor chip.