Figure 1.

(A) Amino acid sequence of DD12 (Os-RPA1). The lightly shaded amino acid sequences comprise SBD-A and SBD-B, the darker shaded sequence the zinc finger domain. The locations of the primers used for in vitro mutagenesis are underlined. (B) Phylogenetic analysis of RPA1 genes from the species indicated. The dendogram was constructed using the clustal method with the PAM250 residue weight table. The accession numbers are: Q01588 (Xenopus laevis), P27694 (H. sapiens), Z70277 (Drosophila melanogaster), P22336 (Saccharomyces cerevisiae), U59385 (Schizosaccharomyces pombe), S38458 (Crithidia fasciculata), AF009179 (O. sativa), and U41535 (C. elegans).