Figure 2.

Yeast complementation. (A) Schematic representation of the chimeric protein encoded by pDS9 containing the rice SBD-A and SBD-B domains (shaded) between the yeast N-terminal and C-terminal regions. The numbers above the diagram represent the amino acid positions of Os-RPA1. (B) Transformed yeast was plated out on complete synthetic medium (minus leucine, plus 5-fluoroorotic acid). Sector a, SBY102 nontransformed; sector b, SBY102 transformed with pDS1, which contains the wild-type RPA1 of yeast; sector c, SBY102 transformed with pDS9; sector d, SBY102 transformed with pJM241, which contains the wild-type RPA2 of yeast on pRS415 (LEU2).