Figure 5.

In vivo transcription analysis of the sequence upstream of the new exon 1. The −1.5 kb mVDRp and −0.5 kb mVDRp constructs were transfected into ROS 17/2.8 cells (A) or NIH 3T3 cells (B) using lipofectin. NIH 3T3 cells were treated with 10 mM forskolin (forsk) or ethanol (control) for 24 hr. Luciferase activity is expressed in relative luciferase units (RLU). Luciferase activity was normalized to protein content of each sample. Values are average (± SD) of experiments in triplicate. Basal level of luciferase activity obtained with the pGL2b construct in ROS 17/2.8 cells and NIH 3T3 cells are 935 RLU ± 182 and 112 RLU ± 28, respectively.