Figure 1.

Coomassie blue stained 12.5% Tricine SDS–PAGE of pools from DrBCAT purification. Purification fractions containing BCAT activity were collected at each step. All samples were boiled at 373 K for 10 min in 4× sample buffer. Lane 1, pool from total cell lysate in 0.3 M NaCl, 10 mM imidazole and 50 mM NaH₂PO₄ pH 8.0; lane 2, pool from supernatant after centrifugation; lane 3, pool from pellet after centrifugation; lane 4, pool from the first His-tag Ni²⁺–NTA column; lane 5, pool from the second His-tag Ni²⁺–NTA column; lane M, molecular-weight markers (kDa).