Figure 4.

Expression of Snrpn in DNA methyltransferase-deficient mice. RNA samples of normal (+/+), Dnmtase-deficient (−/−), and heterozygous (−/+) E9.5 embryos were obtained from R. Jaenisch (Massachusetts Institute of Technology, Cambridge, MA) and reverse transcribed by reverse transcriptase–PCR (RT-PCR) using oligo(dT) as primer. The RT-PCR products were further amplified by PCR using primers described by Szabo and Mann (19), and primers for β actin were 5′-CAGCTTCTTTGCAGCTCCTT and 3′-TCACCCACATAGGAGTCCTT.