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. 2008 Apr 15;8:61. doi: 10.1186/1471-2180-8-61

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Copyright © 2008 Dreher-Lesnick et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Validation of microarray data and comparison with real time qRT-PCR data. (A) Comparison of real time (white bars) and microarray (black bars) fold change results for 14 select R. typhi genes listed in Additional file 6. Fold change ratios represent the difference in transcript abundance/signal for these genes post temperature shift from 37°C to 25°C. (B) Correlation analysis of microarray and real time transcript measurements for the 14 select R. typhi genes mentioned above. The real time qRT-PCR log2 values were plotted against the microarray log₂values. The correlation coefficient (R²) between the two datasets is 0.8227.