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Displacement of [3H]-LY341495 binding to mGluR7 LBD with l-glutamate. The indicated concentrations of glutamate with 24 nM [3H]-LY341495 were incubated with purified mGluR7 LBD (0.65 µg). Each point shows the mean ± standard deviation. The displacement curve and K i value of 130 µM (95% confidence interval = 56.9–297 µM) were obtained by sigmoidal fitting using Prism 3 (GraphPad Software).
