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TTR G53S/E54D/L55S was purified as described in the text and then analyzed by native PAGE before (lane 1) and after (lane 2) heat treatment at 328 K for 96 h (a). In (b), the dissolved precipitates (lane 1) and the soluble fraction remaining in solution (lane 2) after heat treatment were analyzed by SDS–PAGE. The figure shows that heat treatment precipitates heterogenic and oligomeric forms of TTR.
