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. 2008 May 1;22(9):1159–1173. doi: 10.1101/gad.1657408

Figure 1.

Figure 1.

Generation and phenotypic characterization of p65–S276A (RRPA) knock-in mice. (A) Strategy to generate p65–S276A (RRPA) knock-in mice by homologous recombination. A PvuI restriction enzyme site was coupled to the mutation in the targeted allele for PCR analysis. Restriction enzyme sites for HindIII and XbaI and the probes used for Southern blot analysis were as indicated. (B) Sequencing of p65 cDNA obtained by RT–PCR from wild-type and RRPA homozygous mice. (C) Genotype analysis of offspring derived from RRPA heterozygous parents. The number of live embryos displaying an eye phenotype is indicated in parentheses. (D) Eye development abnormalities in RRPA homozygous embryos at 12.5 dpc. (E) Variegated developmental defects in RRPA homozygous embryos. Embryos at 13.5 dpc are shown, as well as wild-type and RRPA heterozygous control embryos. (F) Eye development abnormalities in RRPA homozygous embryos at 15.5 dpc. The embryo presented in the middle panels has a normal-sized eye on the right side and no discernible eye on the left side. The embryo in the right panels has two small eyes.