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. 2008 May 1;22(9):1159–1173. doi: 10.1101/gad.1657408

Figure 5.

Figure 5.

RRPA mutant p65 affects Pax6 gene expression through epigenetic mechanisms. (A) κB-binding sites on/around the Pax6 gene on chromosome 2 in the mouse genome. (B) Binding of p65 to κB sites upstream of the Pax6 gene. ChIP assay was performed in MEFs using antibody against p65. (C) HDAC3 and acetylated H3K9 levels at the κB sites upstream of the Pax6 gene were determined by ChIP assay. (D) Impaired Pax6 expression in RRPA homozygous MEFs was rescued by TSA. MEFs were treated with TSA for 18 h and Pax6 expression was determined by semiquantitative RT–PCR. (E) Impaired Pax6 expression in p65-deficient 3T3 cells stably transfected with RRPA mutant was rescued by TSA. Cells were treated with TSA for 18 h and Pax6 expression was determined by semiquantitative RT–PCR. (F) Analysis of histone modifications on Pax6 promoters P0 and P1 using ChIP assays.