Figure 6.
Pol I occupancy over the IGS1 region is increased by depletion of Rat1. (A) rDNA schematic. Horizontal bars above the rDNA denote the locations of products amplified by real-time PCR (see also Supplemental Table S3). Labels are as in Figure 2A. (B–D) Profiles of Pol I cross-linking over the intergenic region of rDNA repeats. Aliquots from strains PMET3-RAT1 (B); PMET3-RAT1, xrn1Δ (C); and PMET3-RAT1, fob1Δ (D) were taken at 0 and 12 h of Rat1 depletion and processed for Pol I ChIP (Rpa34-13MYC). QPCR values were corrected for background as described in the Materials and Methods and normalized to 25S1, which was set arbitrarily to 1, in order to compensate for differences in immunoprecipitation efficiencies. The mean of three independent experiments is shown with standard error.