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. 2008 May 2;283(18):11905–11912. doi: 10.1074/jbc.M710557200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — The PH domain of AIP1 is necessary but not sufficient to activate IRE1-JNK/XBP-1 signaling. AIP1-KO MLEC were reconstituted with AIP1-F, AIP1-ΔPH, or AIP1-PH by electroporation transfection. Reconstituted MLEC were treated with Tm for the indicated times (0, 30, and 240 min); phosphorylation of IRE1, JNK, and PERK as well as expression of the spliced XBP-1 were determined by Western blot with respective antibodies. Expression of AIP1 proteins was determined by Western blot with anti-FLAG. ns, nonspecific band.