TABLE 1.
Reconstruction statistics for maps of the Φ6 procapsids and the occupancy of the RNA-dependent RNA polymerase, P2, based on integrated density or comparative 35S labeling
| Reconstructiona | P1247 | P1′247 | P147 | P124 |
|---|---|---|---|---|
| Micrograph pairs | 25 | 23 | 18 | 17 |
| Pixel size (Å/pixel) | 2.54 | 2.54 | 2.54 | 2.54 |
| Defocus (μm) | 0.9-1.7 | 0.9-1.7 | 1.0-2.3 | 1.3-2.6 |
| Particles used | 3150 | 902 | 1072 | 1238 |
| Resolution (Å)b | 14 | 19 | 11 | 16 |
| P2 occupancyc | ||||
| Density | 1-2 | 10 | 0 | 3-4 |
| 35S labeling | 3-4 | 7-8 | 0 | 3 |
a
The digits indicate the proteins present. P1'247 is a mutant in P1.
b
Based on Fourier shell correlation at a cut-off of 0.3.
c
The values indicate the corresponding number of P2 monomers/procapsid.