Hypoxia (18%) does not affect caspase-3 activation, APP processing, nor
Aβ generation in H4-APP cells. A, hypoxia treatment alone
(lanes 4–6) does not induce caspase-3 cleavage (activation) as
compared with control conditions (lanes 1–3) in H4-APP cells.
There is no significant difference in amounts of β-actin in control
conditions or hypoxia-treated H4-APP cells. B, quantification of the
Western blot shows that hypoxia treatment (black bar) does not
increase caspase-3 activation compared with control conditions (white
bar)(p = 0.16, NS), normalized to β-actin levels.
C, hypoxia (lanes 4–6) does not affect APP processing
as compared with control conditions (lanes 1–3) in H4-APP
cells. There is no significant difference in amounts of β-actin in
control conditions or hypoxia-treated H4-APP cells. D, quantification
of the Western blot shows that hypoxia treatment (black bar) does not
alter levels of FL-APP as compared with control conditions (white
bar) (p = 0.85, NS), normalized to β-actin levels.
E, quantification of the Western blot shows that hypoxia treatment
(black bar) does not alter levels of APP-CTFs as compared with
control conditions (white bar)(p = 0.24, NS), normalized to
β-actin levels. F, hypoxia (black bar) does not
increase generation of Aβ40 as compared with control conditions
(white bar)(p = 0.45, NS).