TABLE 2.
Residue by residue comparison of HSQC 1H and 13C chemical shifts for the RPS of S. gordonii 38 and mutant strains
| Strain | Residue | H-1, C-1 | H-2, C-2 | H-3, C-3 | H-4, C-4 | H-5, C-5 | H-6, H-6′, C-6 | NAc, CH3 |
|---|---|---|---|---|---|---|---|---|
| Sg 38a | GalNAcα (A) | 5.180, 95.81 | 4.233, 50.91 | 3.973, 68.00 | 4.078, 68.80 | 4.330, 70.44 | 4.01, 4.07, 65.18 | 2.087, 23.12 |
| GC27 | GalNAcα (A) | 5.071, 95.13 | 4.224, 50.41 | 4.012, 68.08 | 4.063, 68.67 | 4.353, 70.33 | 3.997, 4.039, 65.05 | 2.054, 22.72 |
| GC32 | Galα (A) | 5.127, 96.67 | 3.851, 69.13 | 3.963, 70.11 | 4.051, 69.72 | 4.344, 70.50 | 3.980, 4.024, 65.42 | |
| GC51 | Galα (A) | 5.148, 97.18 | 3.903, 68.77 | 3.930, 69.89 | 4.068, 69.52 | 4.339, 70.37 | 3.88, 3.96, 64.78 | |
| GC39 | GalNAcα (A) | 5.070, 95.50 | 4.225, 50.58 | 4.015, 68.35 | 4.066, 68.94 | 4.354, 70.70 | 3.996, 4.042, 65.42 | 2.057, 23.04 |
| GC35 | Galα (A) | 5.127, 96.67 | 3.855, 69.13 | 3.963, 70.11 | 4.051, 69.72 | 4.348, 70.50 | 3.980, 4.026, 65.42 | |
| Sg 38 | Rhaβ (B) | 4.925, 100.51 | 4.332, 73.05 | 3.727, 79.63 | 3.546, 71.75 | 3.476, 73.42 | 1.351, 17.70 | |
| GC27 | Rhaβ (B) | 4.844, 101.13 | 4.207, 68.08 | 3.658, 78.33 | 3.428, 71.06 | 3.431, 72.72 | 1.339, 17.47 | |
| GC32 | Rhaβ (B) | 4.888, 101.36 | 4.290, 68.35 | 3.695, 78.70 | 3.453, 71.28 | 3.458, 73.04 | 1.344, 17.77 | |
| GC51 | Rhaβ (B) | 4.932, 101.76 | 4.371, 73.64 | 3.781, 79.11 | 3.525, 71.67 | 3.482, 73.43 | 1.355, 17.45 | |
| GC39 | Rhaβ (B) | 4.845, 101.55 | 4.210, 68.35 | 3.659, 78.70 | 3.424, 71.28 | 3.434, 73.04 | 1.342, 17.77 | |
| GC35 | Rhaβ (B) | 4.888, 101.36 | 4.295, 68.35 | 3.692, 78.70 | 3.455, 71.28 | 3.460, 73.04 | 1.344, 17.77 | |
| Sg 38 | Glcβ (C) | 4.489, 103.53 | 3.341, 74.02 | 3.596, 76.83 | 3.604, 78.07 | 3.490, 75.60 | 3.783, 3.947, 61.80 | |
| GC27 | Glcβ (C) | 4.512, 103.38 | 3.336, 73.79 | 3.650, 76.04 | 3.648, 77.16 | 3.533, 75.21 | 3.826, 3.943, 61.34 | |
| GC32 | Glcβ (C) | 4.510, 103.70 | 3.346, 74.21 | 3.660, 76.36 | 3.656, 77.53 | 3.536, 75.58 | 3.839, 3.941, 61.71 | |
| GC51 | Glcβ (C) | 4.497, 103.74 | 3.353, 74.04 | 3.612, 76.90 | 3.618, 78.15 | 3.489, 75.67 | 3.783, 3.958, 61.76 | |
| GC39 | Glcβ (C) | 4.613, 102.92 | 3.322, 73.82 | 3.656, 76.17 | 3.649, 77.53 | 3.551, 75.58 | 3.847, 3.942, 61.71 | |
| GC35 | Glcβ (C) | 4.615, 102.92 | 3.323, 73.82 | 3.665, 76.16 | 3.658, 77.53 | 3.538, 75.58 | 3.841, 3.941, 61.52 | |
| Sg 38 | Galfβ (D) | 5.068, 108.66 | 4.070, 81.78 | 4.080, 77.53 | 3.989, 83.93 | 4.010, 70.51 | 3.741, 4.053, 72.08 | |
| GC27 | Galfβ (D) | 5.061, 108.36 | 4.070, 81.51 | 4.070, 77.41 | 3.987, 83.61 | 4.014, 70.23 | 3.748, 4.053, 71.79 | |
| GC32 | Galfβ (D) | 5.059, 108.78 | 4.071, 81.83 | 4.085, 77.73 | 3.990, 83.98 | 4.017, 70.50 | 3.751, 4.051, 72.26 | |
| GC51 | Galfβ (D) | 5.067, 108.85 | 4.072, 81.87 | 4.086, 77.55 | 3.995, 84.01 | 4.010, 70.48 | 3.749, 4.061, 72.20 | |
| GC39 | Galfβ (D) | 5.089, 108.58 | 4.247, 80.46 | 4.281, 85.34 | 4.132, 83.00 | 3.954, 71.28 | 3.681, 3.722, 63.67 | |
| GC35 | Galfβ (D) | 5.088, 108.58 | 4.246, 80.46 | 4.280, 85.34 | 4.131, 83.00 | 3.953, 71.28 | 3.680, 3.716, 63.67 | |
| Sg 38 | GalNAcβ (E) | 4.657, 104.01 | 3.956, 53.37 | 3.745, 71.63 | 3.944, 68.78 | 3.815, 74.58 | 3.746, 3.913, 68.03 | 2.045, 23.16 |
| GC27 | Galβ (E) | 4.527, 105.09 | 3.526, 71.16 | 3.638, 73.06 | 3.929, 69.35 | 3.831, 74.28 | 3.760, 3.894, 67.88 | |
| GC32 | Galβ (E) | 4.527, 105.46 | 3.524, 71.48 | 3.634, 73.43 | 3.924, 69.72 | 3.822, 74.60 | 3.753, 3.895, 68.16 | |
| GC51 | GalNAcβ (E) | 4.645, 104.18 | 3.947, 53.34 | 3.751, 71.62 | 3.944, 68.80 | 3.829, 74.62 | 3.790, 3.906, 68.20 | 2.045, 23.16 |
| GC39 | Galβ (E) | 4.533, 105.46 | 3.529, 71.48 | 3.639, 73.43 | 3.932, 69.52 | 3.834, 74.21 | 3.761, 3.893, 67.77 | |
| GC35 | Galβ (E) | 4.529, 105.46 | 3.526, 71.48 | 3.636, 73.43 | 3.926, 69.52 | 3.826, 74.21 | 3.755, 3.897, 67.77 | |
| Sg 38 | Galα (F) | 5.495, 96.58 | 3.895, 68.02 | 3.964, 79.68 | 4.242, 70.05 | 4.128, 72.38 | 3.73, 61.89 | |
| GC27 | GalNAcα (F) | 5.486, 94.93 | 4.375, 49.23 | 4.078, 77.26 | 4.273, 69.15 | 4.163, 72.43 | 3.780, 3.758, 61.78 | 2.054, 22.72 |
| GC32 | GalNAcα (F) | 5.489, 95.30 | 4.371, 49.60 | 4.085, 77.73 | 4.273, 69.52 | 4.158, 72.65 | 3.775, 3.761, 62.10 | 2.047, 23.04 |
| GC51 | Galα (F) | 5.496, 96.73 | 3.895, 68.80 | 3.969, 79.68 | 4.247, 70.12 | 4.128, 72.40 | 3.73, 61.92 | |
| GC39 | GalNAcα (F) | 5.487, 95.30 | 4.374, 49.41 | 4.074, 77.92 | 4.274, 69.33 | 4.159, 72.65 | 3.778, 3.756, 62.10 | 2.057, 23.04 |
| GC35 | GalNAcα (F) | 5.486, 95.30 | 4.370, 49.41 | 4.070, 77.92 | 4.273, 69.33 | 4.156, 72.65 | 3.777, 3.760, 62.10 | 2.047, 23.04 |
| Sg 38 | Rhaα (G) | 5.040, 100.92 | 4.041, 71.29 | 3.850, 71.10 | 3.449, 72.75 | 4.046, 69.47 | 1.265, 17.49 | |
| GC51 | Rhaα (G) | 5.274, 101.58 | 4.084, 71.06 | 3.857, 71.00 | 3.441, 72.88 | 4.070, 69.46 | 1.278, 17.31 |
a
Sg 38 denotes type 2Gn RPS from S. gordonii 38 (9).