FIGURE 7.
PGC1α regulates MEF2A transcription from the adjacent MEF2 and NRF1 promoter elements. A, HeLa cells were transfected as described for Fig. 4G with p2MEF2A-Luc and expression vectors for MEF2A α2/β and NRF1ΔN86 as indicated. Values are normalized to the activity of the reporter in the absence of co-transfection (= 1.0). Averages from three independent experiments are shown. B, C2C12 cells were transfected with S-MEF2Ap2-Luc reporters containing wild-type (open symbols) or mutated (solid symbols) MEF2A (19) and NRF1 elements, with or without pCDNA-PGC1α, and analyzed as in A, except that activities were normalized to wild-type promoter-reporter (= 100%).