FIGURE 2.

Additive effect of Pkd1 and Runx2-II deficiency on embryonic bone development. A, gross appearance and body weight of newborn mice. Body weight (expressed in mg) represents the mean ± S.E. from four to five individual newborn mice per genotype. Values sharing the same superscript (a or b) are not significantly different at p < 0.05. B, Alizarin red/Alcian blue staining of wild-type, Runx2-II^+/-, Pkd1^+/m1Bei, and double heterozygous Pkd1^+/m1Bei/Runx2-II^+/- newborn mice. Calcified tissues are stained red, and cartilage is stained blue. Panels I-V, respectively, represents a superior view of the skull, clavicles, hyoid bone, sternum and ribs, scapula and forelimb (left image), and caudal vertebrae (right image). Arrows indicated delayed ossification of specific bones.