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. 2008 May 2;283(18):11972–11980. doi: 10.1074/jbc.M800114200

FIGURE 2.

FIGURE 2.

Product inhibition steady-state kinetics. Double reciprocal plots of 1/k versus 1/[ATP] performed at 25 °C. A, ADP product inhibition was measured using (i) varying [ATP] = 5, 10, 15, 20, 30, 50, and 100 μm, (ii) saturating fixed [Tide] = 200 μm, and (iii) [ADP] = 0 μm (•), 5 μm (○), 10 μm (▴), 15 μm (▵), and 20 μm (▾). Solid lines were generated using the kinetic constants determined from the global fit of the data to Equation S23 and its reciprocal Equation S26. B, pTide product inhibition was measured using (i) varying [ATP] = 0.5, 1, 2, 3, 5, 10, 15, and 20 μm, (ii) saturating fixed [Tide] = 200 μm, and (iii) [pTide] = 0 μm (•), 100 μm (○), 200 μm (▴), 300 μm (▵), and 400 μm (▾). Solid lines were generated using the kinetic constants determined from the global fit of the data to Equation S31 and its reciprocal Equation S34.